Stabilization to 32P decay and onset of DNA replication of T4 bacteriophage

摘要: The genetic loci of T4 bacteriophage become susceptible to mutagenesis by the thymidine analog 5-bromodeoxyuridine a few minutes after start intracellular phage growth. This susceptibility presumably signals onset DNA replication. In order examine whether stabilization decay inactivation which develops at growth highly 32 P-labeled phages derives from appearance replicas infecting viral DNA, time course and sensitivity has been followed in one same population T4-infected bacteria. results these experiments are: 1. Both r II q 5-bromodeoxyuridineinduced between 5th 6th min Since two are separated approximately half known map T4, it appears that different parts genome begin their replication very nearly time. 2. infective centers develop more than ultimate stability P before mutagenesis, i.e. synthesis. Stabilization cannot, therefore, result DNA. 3. If synthesis protein is blocked presence antibiotic chloramphenicol outset growth, development both prevented. If, however, only added 3 centors can attain an appreciable while still held abeyance. demonstrates once precedes be consequence vegetative copies