The Use of Three Long Non-Coding RNAs as Potential Prognostic Indicators of Astrocytoma
作者： Feng Zhi , Qiang Wang , Lian Xue , Naiyuan Shao , Rong Wang
DOI: 10.1371/JOURNAL.PONE.0135242
关键词:
摘要: Long noncoding RNAs (lncRNAs) are pervasively transcribed and play a key role in tumorigenesis. The aim of the study was to determine lncRNA expression profile astrocytomas assess its potential clinical value. We performed three-step analysis establish for astrocytoma: a) examined on 3 as well NATs (normal adjacent tissues) using microarray; b) top-hits were validated 40 (WHO grade II-IV) by quantitative real time-PCR (qRT-PCR); c) hits with significant differences re-evaluated qRT-PCR 90 astrocytomas. Finally, 7 lncRNAs found have significantly different astrocytoma samples compared NAT samples. Unsupervised clustering further revealed 7-lncRNA differentiate between tumors upregulation ENST00000545440 NR_002809 associated advanced stages astrocytoma. Using Kaplan-Meier survival analysis, we showed that low BC002811 or XLOC_010967, high poor patient survival. Moreover, Cox proportional hazard regression this prognostic impact independent other clinicopathological factors. Our results indicate may be biomarker prediction post-surgical outcomes.
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Kailiang Zhang, Xiaotian Sun, Xuan Zhou, Lei Han, Luyue Chen, Zhendong Shi, Anling Zhang, Minhua Ye, Qixue Wang, Chaoyong Liu, Jianwei Wei, Yu Ren, Jingxuan Yang, Jianning Zhang, Peiyu Pu, Min Li, Chunsheng Kang, Long non-coding RNA HOTAIR promotes glioblastoma cell cycle progression in an EZH2 dependent manner. Oncotarget. ,vol. 6, pp. 537- 546 ,(2015) , 10.18632/ONCOTARGET.2681
David N. Louis, WHO classification of tumours of the central nervous system International Agency for Research on Cancer. ,(2007)
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Xiaoyong Qin, Zhiwen Zhang, Jie Yao, Peiliang Geng, Jinghui Xue, Xiangping Fu, LncRNA TSLC1-AS1 is a novel tumor suppressor in glioma. International Journal of Clinical and Experimental Pathology. ,vol. 7, pp. 3065- 3072 ,(2014)
Yi-ling Fan, Lei Chen, Ji Wang, Qing Yao, Jie-qing Wan, Over expression of PPP2R2C inhibits human glioma cells growth through the suppression of mTOR pathway FEBS Letters. ,vol. 587, pp. 3892- 3897 ,(2013) , 10.1016/J.FEBSLET.2013.09.029
Hosung Sim, Bin Hu, Mariano S. Viapiano, Reduced Expression of the Hyaluronan and Proteoglycan Link Proteins in Malignant Gliomas Journal of Biological Chemistry. ,vol. 284, pp. 26547- 26556 ,(2009) , 10.1074/JBC.M109.013185
Xiaoqin Zhang, Stella Sun, Jenny Kan Suen Pu, Anderson Chun On Tsang, Derek Lee, Venus On Ying Man, Wai Man Lui, Stanley Thian Sze Wong, Gilberto Ka Kit Leung, Long non-coding RNA expression profiles predict clinical phenotypes in glioma. Neurobiology of Disease. ,vol. 48, pp. 1- 8 ,(2012) , 10.1016/J.NBD.2012.06.004
Xiao-Qin Zhang, Stella Sun, Kwok-Fai Lam, Karrie Mei-Yee Kiang, Jenny Kan-Suen Pu, Amy Shuk-Wai Ho, Wai-Man Lui, Ching-Fai Fung, Thian-Sze Wong, Gilberto Ka-Kit Leung, A long non-coding RNA signature in glioblastoma multiforme predicts survival. Neurobiology of Disease. ,vol. 58, pp. 123- 131 ,(2013) , 10.1016/J.NBD.2013.05.011
Ping Wang, Yun-hui Liu, Yi-long Yao, Zhen Li, Zhi-qing Li, Jun Ma, Yi-xue Xue, Long non-coding RNA CASC2 suppresses malignancy in human gliomas by miR-21☆ Cellular Signalling. ,vol. 27, pp. 275- 282 ,(2015) , 10.1016/J.CELLSIG.2014.11.011
Jie Yao, Baosheng Zhou, Jian Zhang, Peiliang Geng, Kang Liu, Yunfeng Zhu, Weijie Zhu, A new tumor suppressor LncRNA ADAMTS9-AS2 is regulated by DNMT1 and inhibits migration of glioma cells Tumor Biology. ,vol. 35, pp. 7935- 7944 ,(2014) , 10.1007/S13277-014-1949-2