Association of cyclin A and cdk2 with SV40 DNA in replication initiation complexes is cell cycle dependent.

摘要: The cell cycle is driven by the sequential activation of a family cyclin-dependent kinases (CDK) in association with cyclins. In mammalian cells timing cyclin A-associated kinase activity coincides onset DNA synthesis S-phase. Using vitro replication SV40 origin-containing as model system, we have analyzed proteins associated during initiation S-phase extracts. This analysis reveals that, addition to proteins, A and cdk2 are also specifically DNA. regulated occurs presence plasmid T antigen (the viral initiator protein). interactions among involved play an important role replication. We therefore investigated ability associate proteins. Under conditions, from extracts antigen. Further, interaction A-cdk2 mediated via A, purified recombinant associates directly Taken together, our results suggest that components complex, protein-protein between may facilitate complex.