Dissociation in expression of MB1/MT1 and DR1 alloantigens in mutants of a lymphoblastoid cell line.

摘要: Cytotoxicity tests with alloantisera were used to study the expression of HLA-D region antigens in HLA-DR-null mutants a human lymphoblastoid cell line. The initial line contained just one copy MHC as haplotype that included DR1 and MB1/MT1. Gamma ray mutagenesis single cells followed by selection complement an anti-DR monoclonal antibody then isolate DR-null mutants. Two categories identified panel alloantisera. Expressions MB1/MT1 simultaneously lost four Nine still expressed but had DR1. dissociated loss is evidence for separate genetic control these alloantigens. methods exemplify versatile approach conveniently inducing separations closely linked loci MHC.