Identification of Signaling Pathways Targeted by the Food Contaminant FB1: Transcriptome and Kinome Analysis of Samples from Pig Liver and Intestine

摘要: Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium species. In mammals, this toxin causes widespread organ-specific damage; it promotes hepatotoxicity, immunotoxic, alters intestinal functions… Despite its inhibitory effect on de novo ceramide synthesis, molecular mechanism of action and toxicity are not totally elucidated. To explore the FB1 toxicity, we analyzed transcriptome kinome two organs targeted FB1: liver jejunum. Pigs were fed for 4 weeks control diet or FB1-contaminated (10 mg/kg). As expected, FB1-exposed pigs gained less weight displayed higher sphinganine/sphingosine ratio. Comparison transcriptomes kinomes treated versus showed striking differences. Among disrupted pathways in jejunum, highlight Protein Kinase B (AKT) / Phosphatase tensin homolog (PTEN) at intersection FB1-modulated pathways. Most effects mediated regulation level, which influences protein phosphatase 2 (PP2A) phosphoinositide 3-kinase (PI3K)/AKT signaling pathway. This pathway might be new target to counteract toxic Fumonin most spread nutritional's contaminant world. article protected copyright. All rights reserved.