Testicular steroidogenesis in the Cebus monkey throughout postnatal development.

摘要: There is scant information on testicular steroidogenesis during postnatal development in monkeys, particularly New World species. Our purpose was to study the vitro steroidogenic capacity of Cebus monkey testis from birth advanced puberty. Fresh tissue incubated Medium 199 with or without hCG (10 IU/ml); and levels pregnenolone (P 5 ), dehy droepiandrosterone (DHA), progesterone 4 androstenedione (A), testosterone (T), dihydrotestosterone (DHT), 3α-androstanediol (3α-DIOL) were meaured tisue incubation media by RIA. To determine predominant pathway, ratio between concentrations 5-ene 4-ene T precursors determined, relative conversion 3 H-P , 14 C-P calculated. The number Leydig cells per determined all experimental groups. testes monkeys could produce addition gonadotropins at ages. DHA) main steroids found within throughout development. content cell increased continuously age, but concentration reached maximal early puberty did not change thereafter. ratios ranged 2.8 13.2, which suggested a predominance delta-5 pathway. This confirmed finding that more efficiently converted than . production its progressively reach minimal values late 5α-reduced metabolites (DHT 3α-DIOL) low maturation. Addition hGC stimulated steroid infantile pubertal animals. After stimulation, pathway remained predominant, efficiency levels, 5α-reductase activity decreased We conclude 1) produces basal conditions, this age because parallel increment numbers ability cells; 2) acute responses are puberty, coincidence lowest mature testis; 3) 6) attained puberty; 5) ages; has maturation-like effect function