Morphometric study of the testicular interstitial tissue of the monkey Cebus apella during postnatal development

摘要: Abstract The purpose of this study was to evaluate the developmental changes Leydig cells and their precursors during postnatal development in monkey Cebus apella . Four groups monkeys were studied: neonatal, infantile, early pubertal late pubertal. Light microscopy, immunocytochemistry, electron microscopy stereological studies performed determine cytologic cytochemical characteristics, volume density, absolute cell counts cells. In interstitial tissue two components recognized: specific interstitium comprising mature immature differentiating precursors, non-specific including connective blood vessels. Mature polygonal with a round, euchromatic nucleus abundant cytoplasm. Immature more elongated showed heterochromatin. either pale- or dark-stained Pale smooth endoplasmic reticulum (SER), mitochondria tubular cristae glycogen granules. SER dark consisted flat cisternae, only few inclusions lipid droplets. All intensely reactive for 3β-hydrohysteroid dehydrogenase (3β-HSD). Some peritubular acquired nuclear cytoplasmic characteristics that indicated they cells, as evidenced by strong 3β-HSD positivity found scattered tissue. Absolute increased from birth end puberty due an increment numbers size. populations different evolution development. While 7-fold neonatal period at lower rate puberty, remained stable until significantly