Effect of L-Lysine α-Oxidase on Growth of Mouse Leukemic Cells

摘要: L-Lysine α-oxidase, a new fungal enzyme that catalyzes the a-oxidative deamination of L-lysine, inhibited growth L5178Y mouse leukemic cells in vitro. Complete inhibition was observed at concentration 3.3mU/ml, and which induces 50% cell 1mU/ml. DNA synthesis more strongly by treatment than protein RNA syntheses were. The also shown to have antitumor activity against L1210 leukemia vivo. Intraperitoneal injection dose 70 units/kg/day on day I 5 resulted an increase lifespan 34 48 %. reduced L-lysine level both RPMI 1640 medium plasma BDF1 mice. half-life time about 2 hr. In addition growth-inhibitory effect based enzymatic depletion cytotoxic H2O2 formed enzymatically contributed