Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment

摘要: The genus Streptomyces under phylum actinobacteria has been recognized as a prolific source for the production of bioactive secondary metabolites. An actinobacterial strain designated DST103 isolated from wetland fresh water sediment Tamdil Lake, Mizoram, Northeast, India was identified cyaneofuscatus (KY287599) using 16SrRNA gene sequencing which shares 99.87% sequence similarity with NRRL B-2570T. showed broad spectrum antimicrobial activities against Gram negative bacteria (Escherichia coli MTCC 739 and Pseudomonas aeruginosa 2453), positive (Micrococcus luteus NCIM 2170 Staphylococcus aureus 96) yeast pathogen Candida albicans 3017). methanolic extract exhibited highest activity E. (IC50= 2.10 µg/mL) minimum S. 43.63 µg/mL). Five antibiotics [trimethoprim (18μg/g), fluconazole (6μg/g), ketoconazole nalidixic acid (135μg/g), rifampicin (56μg/g)] were detected quantified ultra-performance liquid chromatography (UPLC-ESI-MS/MS). Further, biosynthetic potential genes [polyketide synthases (PKS) type II, non-ribosomal peptide synthetases (NRPS) aminodeoxyisochorismate synthase (phzE)] also in may possibly be responsible compounds. Additionally, gas chromatography-mass spectrometry (GC-MS) analysis presence four volatile compounds might their diverse biological activity. present study revealed DST103, promising resource discovery novel metabolites wide range pathogens.