Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors.

摘要: Angiotensin II acts on at least two distinct receptor subtypes (AT1 and AT2). Most known effects of angiotensin in adult tissues are attributable to the AT1 receptor. The function AT2 is undefined, but its abundant expressions fetal tissues, immature brain, skin wound, atretic ovarian follicles suggest a role growth development. Previous studies suggested that may not be G protein-coupled. Here, from rat fetus expression library, we cloned cDNA encoding unique 363-amino acid protein with pharmacological specificity, tissue distribution, developmental pattern It 34% identical sequence receptor, sharing seven-transmembrane domain topology. A review prior data other receptors suggests this belong class (including somatostatin SSTR1, dopamine D3, frizzled Fz) for which coupling has been demonstrated. All members exhibit and/or neuronal-specific expression. conserved motif third intracellular loop, distinguishing "classical" protein-coupled receptors, mediate novel effects.