Double-immunolabeling systems for phenotyping of immune cells harboring bovine viral diarrhea virus.
作者： H Bielefeldt Ohmann
DOI: 10.1177/35.6.3033062
关键词:
摘要: Optimal staining conditions were defined for simultaneous detection of bovine viral diarrhea virus (BVDV) and mononuclear leukocyte surface antigens in tissue sections cytospins. Because the extreme lability variable stability epitopes on cell differentiation antigens, cryopreservation had to be used. This method gives slightly sub-optimal preservation morphology. However, specificity sensitivity immunolabeling ensured reliable identification double-labeled cells, i.e., phenotypic virus-infected cells within immune system.
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