Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens.

摘要: To establish a novel in vivo test system for rapid detection of environmental estrogens, an ere-zvtg1: gfp transgenic zebrafish line has been generated. In this line, under control conditions, GFP was exclusively expressed the liver mature adult female fish. Male and larval fish did not express but could be induced to after exposure 17-alpha-ethynylestradiol (EE(2)). Concurrent accumulation zvtg1 mRNAs embryos larvae EE(2) observed, which indicated that expression transgene driven by promoter. Green fluorescence first observed at 53, 74, 100 or 131h post-fertilization (hpf) 100, 10, 1 0.1ng/L from 2 cell stage, respectively. As male zebrafish, green 2, 3, 4 7 days, respectively; as female, increased relatively high concentrations (10 100ng/L). with prolonging time repeatedly removal re-addition EE(2). We also demonstrated other estrogenic compounds, including beta-estradiol (E(2), 0.1microg/L), cadmium chloride (CdCl(2), 10microg/L), zearalenone (50microg/L), estriol (E(3), 1microg/L), diethylstilbestrol (DES, 50ng/L) bisphenol A (BPA, 1mg/L) weakly compounds such nonylphenol (NP, up 10mg/L), non-estrogenic steroid hormones progesterone (up 100mg/L) 17-hydroxysteroid 50mg/L). These data suggest is sensitive specific compounds. Because observed-effect are low those environment times very short, promising candidate monitoring estrogens directly, rapidly easily.