Soluble forms of the interleukin-6 signal-transducing receptor component gp130 in human serum possessing a potential to inhibit signals through membrane-anchored gp130

摘要: The interleukin-6 (IL-6) signal is transduced through membrane-anchored gp130, which associated with IL-6 receptor (IL-6R) in the presence of IL-6. Soluble forms gp130 (sgp130) molecular weights 90 and 110 Kd were found human serum. In recombinant IL- 6 (rIL-6), serum sgp130 capable associating sIL-6R. By sandwich enzyme-linked immunosorbent assay, healthy sera was shown to contain 390 +/- 72 ng/mL sgp130. A mouse pro-B-cell line-derived transfectant, BAF-130, expressing used examine function When supplemented rIL-6, induced DNA synthesis BAF-130 cells, whereas deprived sIL-6R did not. contrast, cells by rIL-6-supplemented increased when These results indicated that could negatively regulate signal. Recently, has been be involved signaling processes oncostatin M, leukemia inhibitory factor, ciliary neurotropic addition those Recombinant showed effect on biologic such cytokines. This work implies physiologic roles naturally produced modulating signals gp130.