Alterations of the actin cytoskeleton and increased nitric oxide synthesis are common features in human primary endothelial cell response to changes in gravity

摘要: Because endothelial cells are fundamental to the maintenance of functional integrity vascular wall, modifications in altered gravity conditions might offer some insights into mechanisms leading circulatory impairment astronauts. We cultured human a dedicated centrifuge (MidiCAR) generate hypergravity and two different devices, namely Rotating Wall Vessel Random Positioning Machine, hypogravity. Hypogravity stimulated growth, did not affect migration, enhanced nitric oxide production. It also remodeled actin cytoskeleton reduced total amounts actin. Hypergravity markedly synthesis. In addition, distribution fibers without, however, affecting A short exposure (8 min) abolished hypogravity induced growth advantage. Our results indicate that cytoskeletal alterations increased production represent common denominators responses both hypergravity.