Detection of HEV antigen as a novel marker for the diagnosis of hepatitis E

摘要: Infection with hepatitis E virus (HEV) may be diagnosed by the presence of HEV RNA or anti-HEV antibodies. An enzyme immunoassay (EIA) was developed for detection antigen. Twenty-four monoclonal antibodies (mAbs) were produced. indirect sandwich EIA to detect antigen using a combination three mAbs as coating Approximately 44.6% (33/74), 28.6% (50/175), and none (0/27) sera positive IgM alone, both IgG, IgG alone also this EIA. Forty-two antibody-positive tested in parallel concordance 81.0% (34/42). All PCR products found belong genotype 4. In order evaluate temporal relationship between positivity RNA, IgM, ALT concentrations, macaques infected genotypes 1 4 serial samples collected. The results showed that can capsid proteins genotypes. detectable prior elevation appearance monkeys lasted several weeks all cases. became serum at almost same time feces but persisted less than RNA. This assay should valuable diagnosis acute E, particularly window period seroconversion anti-HEV.