Preparation of Soluble Proteins from Escherichia coli
作者： Paul T. Wingfield
DOI: 10.1002/0471140864.PS0602S78
关键词:
摘要: Purification of human IL-1β is used in this unit as an example the preparation soluble proteins from E. coli. Bacteria containing are lysed, and IL-1 β resulting supernatant purified by anion-exchange chromatography, salt precipitation cation-exchange then concentrated. Finally, protein applied to a gel-filtration column separate it remaining higher- lower-molecular-weight contaminants, stored frozen or lyophilized. The purification protocol described typical for that expressed fairly high abundance (i.e., >5% total protein) accumulates state. Also, procedure serves how use classical purifications methods which may also be conjunction with affinity-based now more commonly used.
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