Selection and characterization of single-chain recombinant antibodies against phosphoprotein of newcastle disease virus
作者： Benqiang Li , Jiaxin Ye , Yuan Lin , Man Wang , Rui Jia
DOI: 10.1016/J.BIOLOGICALS.2014.05.006
关键词:
摘要: Phosphoprotein (P), involved in virus RNA replication and transcription, had become a new target for the research on treating Newcastle disease (NDV). Here we described cloning expression of phosphoprotein from NDV, then screened anti-P antibodies chicken single chain fragment variable (scFv) library, which were generated chickens immunized with ND vaccines. As first step, recombinant vector pET28a-P was successfully constructed. In following two positive scFv clones library selected by indirect enzyme-linked immunosorbent assay (ELISA) method. The sequence analysis showed that there more variation complementary determine region (CDR) VH VL, CDR3 exhibited significant change amino acid number type. another experiment, purified used shown to be specific NDV-P western blot. results indicated strategy this experiment proved convenient way screening antibody, paved immunization diagnosis exploration integrated control NDV.
sci-hub.se 参考文章(20)
Wayne T. McCormack, Larry W. Tjoelker, Craig B. Thompson, Immunoglobulin gene diversification by gene conversion. Progress in Nucleic Acid Research and Molecular Biology. ,vol. 45, pp. 27- 45 ,(1993) , 10.1016/S0079-6603(08)60865-X
Wouter van Wyngaardt, Teresiah Malatji, Cordelia Mashau, Jeanni Fehrsen, Frances Jordaan, Dubravka Miltiadou, Dion H du Plessis, A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes BMC Biotechnology. ,vol. 4, pp. 6- 6 ,(2004) , 10.1186/1472-6750-4-6
Paul T. Wingfield, Preparation of Soluble Proteins from Escherichia coli Current protocols in protein science. ,vol. 78, ,(1995) , 10.1002/0471140864.PS0602S78
CH Okino, MFSM Montassier, PEN Givisiez, CRAG Furuyama, L Brentano, HJ Montassier, Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR Brazilian Journal of Poultry Science. ,vol. 7, pp. 59- 66 ,(2005) , 10.1590/S1516-635X2005000100010
Shouping Zhang, Xiaoting Wang, Changguang Zhao, Dehua Liu, Yanxin Hu, Jixun Zhao, Guozhong Zhang, Phylogenetic and pathotypical analysis of two virulent Newcastle disease viruses isolated from domestic ducks in China. PLOS ONE. ,vol. 6, ,(2011) , 10.1371/JOURNAL.PONE.0025000
Sherin Smallwood, Kevin W. Ryan, Sue A. Moyer, Deletion Analysis Defines a Carboxyl-Proximal Region of Sendai Virus P Protein That Binds to the Polymerase L Protein Virology. ,vol. 202, pp. 154- 163 ,(1994) , 10.1006/VIRO.1994.1331
Adam J. Foord, Janine D. Muller, Meng Yu, Lin-Fa Wang, Hans G. Heine, Production and application of recombinant antibodies to foot-and-mouth disease virus non-structural protein 3ABC. Journal of Immunological Methods. ,vol. 321, pp. 142- 151 ,(2007) , 10.1016/J.JIM.2007.01.014
Masaki Magari, Yuichi Kanehiro, Kagefumi Todo, Mika Ikeda, Naoki Kanayama, Hitoshi Ohmori, Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repertoire. Biochemical and Biophysical Research Communications. ,vol. 396, pp. 353- 358 ,(2010) , 10.1016/J.BBRC.2010.04.096
S. I. Sapats, H. G. Heine, L. Trinidad, G. J. Gould, A. J. Foord, S. G. Doolan, S. Prowse, J. Ignjatovic, Generation of chicken single chain antibody variable fragments (scFv) that differentiate and neutralize infectious bursal disease virus (IBDV) Archives of Virology. ,vol. 148, pp. 497- 515 ,(2003) , 10.1007/S00705-002-0931-2
F. Jahanshiri, M. Eshaghi, K. Yusoff, Identification of phosphoprotein:phosphoprotein and phosphoprotein:nucleocapsid protein interaction domains of the Newcastle disease virus. Archives of Virology. ,vol. 150, pp. 611- 618 ,(2005) , 10.1007/S00705-004-0439-Z