Thermostable Alkaline Phytase from Alcaligenes sp. in Improving Bioavailability of Phosphorus in Animal Feed: In Vitro Analysis
作者： Ponnuswamy Vijayaraghavan , R. Raja Primiya , Samuel Gnana Prakash Vincent
DOI: 10.5402/2013/394305
关键词:
摘要: A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of included a fermentation period 96 h, pH 8.0, addition 1% (w/v) maltose beef extract to culture medium. This enzyme purified homogeneity had an apparent molecular mass 41 kDa. range temperature activity were found be 7.0-8.0 60°C, respectively. strongly inhibited by 0.005 M Mn2+, Mg2+, Zn2+. In vitro studies revealed that from released inorganic phosphate plant phytates. Phytase 1930 ± 28, 1740 ± 13, 1050 ± 31, 845 ± 7, 1935 ± 32, 1655 ± 21 mg phosphate/kg phytates, namely, chick pea, corn, green groundnut, pearl feed,
hindawi.com参考文章(29)
日本農芸化学会, Agricultural and biological chemistry Agricultural Chemical Society of Japan. ,(1961)
Hertha H. Taussky, Ephraim Shorr, Gloria Kurzmann, A microcolorimetric method for the determination of inorganic phosphorus. Journal of Biological Chemistry. ,vol. 202, pp. 675- 685 ,(1953) , 10.1016/S0021-9258(18)66180-0
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Seong Jun Yoon, Yun Jaie Choi, Hae Ki Min, Kwang Keun Cho, Jin Wook Kim, Sang Cheol Lee, Yeon Hoo Jung, Isolation and identification of phytase-producing bacterium, Enterobacter sp. 4, and enzymatic properties of phytase enzyme Enzyme and Microbial Technology. ,vol. 18, pp. 449- 454 ,(1996) , 10.1016/0141-0229(95)00131-X
Othmar P. Walz, Josef Pallauf, Microbial phytase combined with amino acid supplementation reduces P and N excretion of growing and finishing pigs without loss of performance International Journal of Food Science and Technology. ,vol. 37, pp. 835- 848 ,(2002) , 10.1046/J.1365-2621.2002.00642.X
Ralf Greiner, Abd-ElAziem Farouk, Purification and Characterization of a Bacterial Phytase Whose Properties Make it Exceptionally Useful as a Feed Supplement Protein Journal. ,vol. 26, pp. 467- 474 ,(2007) , 10.1007/S10930-007-9086-Z
H. Fandrejewski, S. Raj, D. Weremko, T. Zebrowska, In K. Han, J. H. Kim, W. T. Cho, Apparent Digestibility of Phosphorus in Experimental Feeds and the Effect of Commercial Phytase Asian-australasian Journal of Animal Sciences. ,vol. 10, pp. 665- 670 ,(1997) , 10.5713/AJAS.1997.665
Jaie Soon Cho, Chang Whan Lee, Seung Ha Kang, Jae Cheon Lee, Jin Duck Bok, Yang Soo Moon, Hong Gu Lee, Sung Chan Kim, Yun Jaie Choi, Purification and characterization of a phytase from Pseudomonas syringae MOK1. Current Microbiology. ,vol. 47, pp. 290- 294 ,(2003) , 10.1007/S00284-002-3966-4
Ann-Sofie Sandberg, Thomas Andlid, Phytogenic and microbial phytases in human nutrition International Journal of Food Science and Technology. ,vol. 37, pp. 823- 833 ,(2002) , 10.1046/J.1365-2621.2002.00641.X
Barbara F. Harland, Eugene R. Morris, Phytate: A good or a bad food component? Nutrition Research. ,vol. 15, pp. 733- 754 ,(1995) , 10.1016/0271-5317(95)00040-P