Quantitative Expression Analysis of APP Pathway and Tau Phosphorylation-Related Genes in the ICV STZ-Induced Non-Human Primate Model of Sporadic Alzheimer’s Disease

摘要: The accumulation and aggregation of misfolded proteins in the brain, such as amyloid-β (Aβ) hyperphosphorylated tau, is a neuropathological hallmark Alzheimer’s disease (AD). Previously, we developed validated novel non-human primate model for sporadic AD (sAD) research using intracerebroventricular administration streptozotocin (icv STZ). To date, no characterization AD-related genes different brain regions has been performed. Therefore, current study, expression seven amyloid precursor protein (APP) pathway-related five tau phosphorylation-related was investigated by quantitative real-time PCR experiments, two matched-pair samples from control icv STZ-treated cynomolgus monkeys. showed similar patterns within groups; however, marked differences gene were observed between groups. Remarkably, other than β-secretase (BACE1) cyclin-dependent kinase 5 (CDK5), all tested models compared to controls, with increased levels precuneus occipital cortex. However, significant changes not detected frontal cortex, hippocampus, or posterior cingulate. Based on these results, conclude that APP may be cleaved via general metabolic mechanisms α- γ-secretase levels, hyperphosphorylation could mediated elevated kinase, specifically