MMP-14 and TIMP-2 Overexpression Protects against Hydroquinone-Induced Oxidant Injury in RPE: Implications for Extracellular Matrix Turnover

摘要: PURPOSE. To investigate whether overexpression of MMP-14 and/or TIMP-2 would overcome the effect nonlethal oxidant injury with hydroquinone (HQ) on MMP-2 activity. METHODS. Human and TIMP2 cDNA were cloned into a mammalian expression vector. Transient transfections performed human ARPE-19 cells. The cells incubated 48 hours after transfection dose HQ for either 6 or 18 then collected protein determination RNA isolation. activity determined by Western blot zymography. extracellular matrix (ECM) components type I IV collagen laminin analyzed analysis real-time PCR. RESULTS. modestly decreased MMP-2. recovered only co-overexpression TIMP-2, but further hours. alone contributed as much to recovery seemed not be altered. Type transcriptional levels remained unaffected, whereas transcripts HQ. Transfection return normal. On other hand, collagens accumulated treatment, an prevented transfection. CONCLUSIONS. contribute maintenance adequate in injury. In addition, changes ECM may result consequence relevant progression dry AMD.