Involvement of Two Specific Causes of Cell Mortality in Freeze-Thaw Cycles with Freezing to −196°C
作者： Frédéric Dumont , Pierre-André Marechal , Patrick Gervais
DOI: 10.1128/AEM.72.2.1330-1335.2006
关键词:
摘要: The purpose of this study was to examine cell viability after freezing. Two distinct ranges temperature were identified as corresponding stages at which yeast mortality occurred during freezing −196°C. upper range related the crystallization medium, dependent on solute concentration; in prevented by high concentrations, and proportion medium vitreous state greater than crystallized state. lower recrystallization that thawing. Mortality increased a cooling rate and/or concentration low (less −70°C). However, thawing range. Overall, it found could be conserved better under conditions increasing osmotic pressure using an warming rate.
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