Oligomeric proanthocyanidins protects A549 cells against H2O2-induced oxidative stress via the Nrf2-ARE pathway.
作者： Chao Sun , Weiguo Jin , Hongcan Shi
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摘要: Abstract Oxidative signaling and oxidative stress contribute to aging, cancer diseases resulting from lung fibrosis. In this study, we explored the anti-oxidative potential of oligomeric proanthocyanidins (OPCs), natural flavonoid compounds. We examined protective effects OPCs against hydrogen peroxide (H2O2)-induced in non-small cell cells (A549). demonstrated that OPC markedly attenuated H2O2-induced A549 viability, as shown by 3-[4,5-dimethylthiazol-2-yl)]-2,5-diphenyl-tetrazolium bromide (MTT) assay. At same time, inhibited significantly increasing activities superoxide dismutase, catalase glutathione, reducing levels reactive oxygen species (ROS) malondialdehyde (MDA). Treatment cells with promoted nuclear translocation NF-E2-related factor 2 (Nrf2) enhanced expression its target genes [heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO1) thioredoxin reductase 1 (TXNRD1)] different fold change values at both mRNA protein level. The knockout Nrf2 using CRISPR/Cas9 technology OPC-mediated ARE gene transcription, almost abolished stress. On whole, our study suggests plays an important role controlling antioxidant response via Nrf2-ARE pathway.
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