A new Multilocus Sequence Analysis Scheme for Mycobacterium tuberculosis
作者： Yi Jiang , Yuan Yuan Zhang , Zhi Guang Liu , Kang Lin Wan , Xiu Qin Zhao
DOI: 10.3967/0895-3988.2012.06.003
关键词:
摘要: Abstract Objective Tuberculosis remains one of the most serious infectious diseases in world. In this study, a scheme Mycobacterium tuberculosis (M. tuberculosis) multilocus sequence analysis (MLSA) was established for phylogenetic and epidemiology analysis. Methods To establish M. MLSA, genome H37Rv, CCDC5079 CCDC5180 were compared, some variable genes chosen to be MLSA typing scheme. 44 clinical isolates typed by IS6110-RFLP, soligotyping, evaluate methods. Results After comparison genome, seven high discrimination gene loci (recX, rpsL, rmlC, rpmG1, mprA, gcvH, ideR) finally. 11 SNP sites those found among isolate strains types (STs) identified. Based on Hunter-Gaston Index (HGI), not as good at strain level but HGI much better than that spoligotyping. addition, MEGA result data similar spoligotyping/PGG lineage, showing strong signal modern tuberculosis. The eBURST revealed 4 (ST) came into main cluster, major clonal complexes strains. Conclusion genotyping only can used molecular typing, also is an ideal method
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