Genetic characterization of Anaplasma phagocytophilum strains from goats (Capra aegagrus hircus) and water buffalo (Bubalus bubalis) by 16S rRNA gene, ankA gene and multilocus sequence typing.

摘要: Anaplasma phagocytophilum is a Gram-negative obligate intracellular bacterium that replicates in neutrophil granulocytes. It transmitted by ticks and causes tick-borne fever domestic ruminants such as sheep, cattle goats. However, contrast to sheep little known about the clinical course of infection We report here on three cases symptomatic with A. two goats (Capra aegagrus hircus) one water buffalo (Bubalus bubalis). The animals showed symptoms laboratory findings similar cattle. To our knowledge, this first buffalos phagocytophilum. infecting strains were genetically characterized 16S rRNA gene, ankA gene multilocus sequence typing (MLST). Four other from asymptomatically infected also included. sequences five part formerly described clusters I IV are contain goat belonged cluster II was be restricted roe deer. A observation made for MLST clustered cattle, whereas found deer cluster. since most distinct strains, might not represent major reservoir hosts ruminants. When differing parts used results conflicting. This shows use standardized method highly desirable generate easily comparable results.