An anti-apoptotic pattern correlates with multidrug resistance in acute myeloid leukemia patients: a comparative study of active caspase-3, cleaved PARPs, Bcl-2, Survivin and MDR1 gene.
作者： Margarita L. Guenova , Gueorgui N. Balatzenko , Vessela R. Nikolova , Branimir V. Spassov , Spiro M. Konstantinov
DOI: 10.1179/102453309X12583347113690
关键词:
摘要: Bone marrow samples of 17 acute myeloid leukemia (AML) patients were analyzed for apoptosis-related markers. The levels active caspase-3 (aC-3), Bcl-2 and cleaved poly(ADP-ribose) polymerase (cPARP) measured by flow cytometry compared with survivin MDR1 gene expression as defined reverse transcriptase chain reaction (RT-PCR). results showed heterogeneous patterns intracellular the studied proteins in AML patients: aC-3 (mean 34.6+/-52.5 U/ml), 3268.4+/-2055.2 cPARPs 24.59+/-29.97 U/ml). Survivin genes overexpressed 9 10 patients, respectively. Patients high mRNA significantly lower (11.8+/-14.3 versus 53.9+/-31.9 U/ml P=0.005) a tendency towards higher (49.3+/-70.0 18.1+/-9.9 overexpression (7/9 3/8 patients), well poorer therapeutic response survival. Our data support potential relevance markers further understanding disease; however, heterogeneity complexity molecular interactions warrants prospective studies.
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