High-throughput assay for bacterial adhesion on acellular dermal matrices and synthetic surgical materials.

摘要: BACKGROUND There has been increasing use of synthetic and acellular dermal matrix materials in surgery, ranging from breast reconstruction to hernia repairs. is a paucity data on how compares with other surgical as substrate for bacterial adhesion, the first step formation biofilm, which occurs prosthetic wound infections. The authors have designed high-throughput assay evaluate Staphylococcus aureus adherence various biologically derived materials. METHODS Clinical isolates S. (strains SC-1 UAMS-1) were cultured different materials, was measured using resazurin cell vitality assay. Four that are commonly used surgery evaluated: Prolene mesh, Vicryl two preparations (AlloDerm FlexHD). able develop reliable quantifying adhesion RESULTS can be reliably quantify material material. strains UAMS-1 both adhered better versus FlexHD) than Prolene. also Strain did strain SC-1. CONCLUSIONS results show adheres more readily provides standard method evaluating regard potential propensity biofilm development.