Effects of compression on the loss of newly synthesized proteoglycans and proteins from cartilage explants.

摘要: Abstract The effects of mechanical compression calf cartilage explants on the catabolism and loss into medium proteoglycans proteins radiolabeled with [35S]sulfate [3H]proline were examined. A single 2- or 12-h 3-mm diameter disks from a thickness 1.25 to 0.50 mm, slow cyclic (2 h on/2 off) mm 1.00, 0.75, for 24 led transient alterations and/or sustained increases in macromolecules. imposing removing loads consistent several compression-induced physical mediators including fluid flow, diffusion, matrix disruption. Cyclic induced convective flow enhanced 35S- 3H-labeled macromolecules tissue medium. In contrast, prolonged static consolidation appeared hinder diffusional transport Since high amplitude increase rate 3H- 35S-labeled that was accompanied by an [3H]hydroxyproline residues hydration, such may have caused disruption collagen meshwork. lost during smaller average size than those controls, but contained similarly low proportion (~15%) could form aggregates excess hyaluronate link protein. distribution aggregability remaining not markedly affected. proteoglycan paralleled This study provides framework elucidating biophysical mechanisms involved redistribution, catabolism, compression.