Ginsenoside Rg1 protects human umbilical cord blood-derived stromal cells against tert-Butyl hydroperoxide-induced apoptosis through Akt–FoxO3a–Bim signaling pathway

摘要: Human umbilical cord blood-derived stromal cells (hUCBDSCs) possess strong capability of supporting hematopoiesis and immune regulation, whereas some stress conditions cause reactive oxygen species (ROS) accumulation then lead to oxidative injury cell apoptosis. Ginsenoside Rg1 (G-Rg1) has been demonstrated exert antioxidative prosurvival effects in many types. In this study, the tert-Butyl hydroperoxide (t-BHP), an analog hydroperoxide, was utilized mimic damage hUCBDSCs. We aimed investigate on protecting hUCBDSCs from t-BHP-induced apoptosis, as well possible signaling pathway involved. It shown that treatment with G-Rg1 markedly restored viability loss, promoted CFU-F formation, inhibited also caused a reduced production LDH MDA while significantly enhancing activity SOD. Mechanistically, phosphorylation Akt FoxO3a led cytoplasmic translocation FoxO3a, which turn suppressed FoxO3a-modulated expression proapoptotic Bim elevated ratio Bcl-2 Bax. All these results suggest enhances survival protects them against apoptosis at least partially through Akt–FoxO3a–Bim pathway.