A homogeneous carbon nanosphere film-spot: For highly efficient laser desorption/ionization of small biomolecules

摘要: Abstract In this work, a novel strategy for highly efficient detection of small molecules with homogeneous carbon nanosphere film-spot (HCNFs) was carried out the first time by negative surface-assisted laser desorption/ionization time-of-flight mass spectroscopy (SALDI-TOF MS). The HCNFs fabricated monolayer oxidized nanospheres (OCS) self-assembling on (LDI) target. With HCNFs, no background interference and exclusive deprotonated ([M−H] − ) peaks analytes were obtained wide range biomolecules including fatty acids, amino nucleobases, traditional Chinese medicines, anti-cancer drugs in SALDI-TOF MS. Comparing other carbon-based matrices as well organic matrix, OCS film exhibited various advantages fewer interfering fragments, stronger signal intensities, higher sensitivity. Moreover, improved spotting homogeneity “sweet-spot” searching LDI target avoided good reproducibility LDI-TOF MS analysis. This study is expanding application providing promising approach rapid analysis none-interference reproducibility.