Affinity chromatography of beta2-microglobulin from human lymphocytes on concanavalin-A-sepharose. Determination of carbohydrate-containing protein molecules complexed with beta2-microglobulin.
作者： T. PLESNER
DOI: 10.1111/J.1365-3083.1976.TB00249.X
关键词:
摘要: Beta2-microglobulin was extracted from human lymphocytes with nonionic detergent and separated by affinity chromatography on concanavalin-A-Sepharose. The retarded part of beta2-microglobulin is assumed to be associated HLA antigens. Using a radioimmunoassay for beta2-microglobulin, the average number presumably free molecules HLA-associated per lymphocyte estimated 3.8 x 10(5) 1.4 10(5), respectively.
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