CLASSIFICATION OF SAUDI ARABIAN CAMEL (CAMELUS DROMEDARIUS) SUBTYPES BASED ON RAPD TECHNIQUE

摘要: The genetic diversity and relationships amongst the dromedary (Camelus dromedarius) populations are poorly documented. This study compares, for first time, variation in DNA fingerprinting of Saudi Arabian camels. objective this was to analyze inter- intra-specific relationship between six widely distributed subtypes belonging three types (Magateer, Magaheem Beedh) One hundred twenty samples were analyzed by random amplified polymorphic (RAPD) methodology using universal decamer primers. All primers produced novel fragments all tested samples. analysis electrophoretic patterns revealed a high polymorphism size, number intensity bands. generated fingerprint specific, i.e., one type could be differentiated from another. There differences dendrograms each subtype other analyzed. However, profiles individuals virtually identical enabling easy distinction subtype. Estimation 120 6 3 camel cluster UPGMA method two main clusters. Cluster A consisted (Magaheem B) with 0.76-0.85 similarity matrix. B (Magateer B, Beedh 0.76-0.83 range. is subdivided into subgroups; Subgroup includes Magateer B. Subtype 73% genetically similar rest subtypes. average among more than 80%. Our results suggest closer B; least related RAPD showed rich heterogeneous which concordance variability observed phenotypic markers. On contrary, A, exhibit homogeneous pattern indicative very low level polymorphism, congruent reduced found these molecular It concluded that, grouping indicated trees reasonably well correlated supported conventional morphological physiological classification criteria. Identification intraspecific suggests that having potential aid identification reproducibility polymorphisms may lead development subtype-specific markers native Arabia.