Clonogenic assays measure leukemia stem cell killing not detectable by chromium release and flow cytometric cytotoxicity assays
作者： Brent A. Williams , Xing-Hua Wang , Armand Keating
DOI: 10.3109/14653241003628167
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摘要: AbstractBackground aims. NK-92, a permanent natural killer (NK) cell line, shows cytotoxicity against variety of tumors and has been tested in phase I trial. We the toxicity NK-92 chemotherapy drugs stem capacity acute leukemia KG1. While chromium-release assay is most common method for assessing immune effectors, flow cytometry increasingly used, relationship either to clonogenic readouts remains unknown. Methods. KG1 was assessed frequency by serial dilution, single-cell sorting colony growth methylcellulose. sorted into CD34+ CD38+ CD38− populations recultured liquid medium or methylcellulose determine proliferative each fraction. Cytotoxicity daunorubicin cytarabine measured using assay, assays. Results. The culture-initiating whole betw...
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