Effect of ABC transporters on HIV-1 infection: inhibition of virus production by the MDR1 transporter

摘要: The MDR1 multidrug transporter P-gp (P-glycoprotein) is an efflux pump that extrudes diverse hydrophobic drugs and peptides from cells. Since the entry of HIV-1 into cells involves initial interaction viral gp41 peptide with plasma membrane, a potential effect on infectivity was explored. Virus production greatly decreased when overexpressed at surface continuous CD4(+) human T-leukemic cell line (12D7) infected HIV-1(NL4-3), T-tropic molecular clone HIV-1. overexpression did not significantly alter expression or distribution either receptor CD4 coreceptor CXCR4. Reduction in P-gp-expressing occurred both during fusion membranes subsequent step(s) life cycle.