Inflammatory mediators regulate cathepsin S in macrophages and microglia: A role in attenuating heparan sulfate interactions.

摘要: Cathepsin S is a member of the family cysteine lysosomal proteases. The distribution cathepsin restricted to cells from mononuclear lineage both in brain and periphery. Also, its protease activity uniquely stable at neutral pH. We compared expression S, B, L mRNAs various undifferentiated differentiated origin, examined modulation these by inflammatory mediators (lipopolysaccharide cytokines). In addition, effect agents on protein levels was also determined. Lastly, ability process basement membrane components such as heparan sulfate proteoglycans vitro vivo assessed. are expressed mature macrophages microglial not monocytes. Activators negatively regulate all three transcripts. Consistent with this, treatment leads decrease intracellular activity. However, same treatments result stimulation secreted capable degrading vitro. when endothelial cells, autocrinely attenuates basic fibroblast growth factor (bFGF)-mediated binding FGF receptor containing acting proteoglycans. Taken together, data imply that regulatable plays role degradation extracellular proteins, whose secretion microglia increased signals lead activation may be important regulating matrix interactions.