Proliferating cell nuclear antigen expression in Wistar rat livers: A retrospective immunohistochemical study of normal and neoplastic livers

摘要: Abstract Formalin-fixed, paraffin-embedded liver specimens from 27 2-year-old Wistar rats, including 10 normal livers, 11 hepatocellular adenomas, 2 carcinomas, and 4 cystic cholangiomas, were immunostained using the streptavidin/peroxidase method PC10 monoclonal antibody (Mab), which recognizes an epitope on proliferating cell nuclear antigen (PCNA). The following PCNA labeling index (LI) mean values found for above four groups of specimens: 0.43 ± 0.31 %; 1.51 +- 0.59 24.80 % 10.28 0.61 0.21 %. Our findings indicate that LI clearly separates malignancies other benign tumors, as well normal, non-neoplastic, tissues. Although seemed to reflect histological grading (i. e. neoplastic benign, malignant), overlapping between livers adenomas was observed in five cases 3 where varied 0.74 0.96 %). It thus appears immunohistochemistry represents a promising tool investigating proliferation laboratory permits distinguishing malignant parenchymal tumors.