Growth fraction estimation of malignant lymphomas in formalin-fixed paraffin-embedded tissue using anti-PCNA/Cyclin 19A2. Correlation with Ki-67 labeling.

摘要: The immunohistochemical detection of PCNA/Cyclin, a nuclear protein associated with cell proliferation, represents potentially useful tool for the study tumor proliferative activity. Previous studies investigating reactivity anti-PCNA/Cyclin monoclonal antibody 19A2 have not clearly defined population proliferating cells which reacts in tissue sections. authors describe method PCNA/Cyclin formalin-fixed, paraffin-embedded using routine biotin-streptavidin system that employs an anti-IgM, mu-chain-specific second-stage antibody. used this to activity 24 malignant lymphomas, consisting 12 low-grade lymphomas (LGLs) and intermediate-grade (IGLs), five reactive tonsils. data was compared Ki-67 labeling frozen sections same group cases. provided easily detectable staining demonstrating varying intensity staining, latter finding most likely due concentration during cycle. In tonsils, reacted germinal center basal keratinocytes. there good correlation between (r = 0.90, P less than 0.001). subgroup LGLs showed mean 26% 28%. IGLs, 54% 59%. These results indicate detects fraction is similar detected by Ki-67, ie, growth fraction, reliable marker uniformly handled, tissue.