Bacterial promoters and methods of use

摘要: Compositions and methods are disclosed herein that relate to the development of fusion promoters for regulating gene expression in bacteria. Embodiments include comprising one or more operators linked a promoter is modified have altered activity Gram-positive organisms. Vectors cells containing these also described. Other embodiments include, using regulate nucleic acid and/or polypeptide expression, identify proliferation-required genes, molecules having potential antibiotic activity.