Heterogeneous nucleation (and adhesion) of lysozyme crystals

作者: Daniela Tsekova , Sybila Dimitrova , Christo N Nanev

DOI: 10.1016/S0022-0248(98)00827-6

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摘要: Abstract The heterogeneous nucleation of hen-egg-white lysozyme (HEWL) crystals has been investigated using a double-(thermal)-pulse technique, thus detaching from the growth stage. n ( t ) dependencies nucleus number, , vs. time, were plotted for surfaces rendered hydrophobic (by means hexamethyl-disilazane) and bare glass surfaces. Preferred crystallite orientation supplied additional information. discussion is based on classical Stranski–Kaischew theory crystal nucleation. With purposely developed new technique adhesion energies have measured HEWL crystals, grown different supports.

参考文章(15)
F. Rosenberger, P.G. Vekilov, M. Muschol, B.R. Thomas, Nucleation and crystallization of globular proteins — what we know and what is missing Journal of Crystal Growth. ,vol. 168, pp. 1- 27 ,(1996) , 10.1016/0022-0248(96)00358-2
A.A. Chernov, Protein versus conventional crystals: creation of defects Journal of Crystal Growth. ,vol. 174, pp. 354- 361 ,(1997) , 10.1016/S0022-0248(96)01129-3
S.D. Durbin, G. Feher, Crystal growth studies of lysozyme as a model for protein crystallization Journal of Crystal Growth. ,vol. 76, pp. 583- 592 ,(1986) , 10.1016/0022-0248(86)90175-2
Franz Rosenberger, Inorganic and protein crystal growth - similarities and differences Journal of Crystal Growth. ,vol. 76, pp. 618- 636 ,(1986) , 10.1016/0022-0248(86)90179-X
Airlie J McCoy, V Chandana Epa, Peter M Colman, None, Electrostatic complementarity at protein/protein interfaces. Journal of Molecular Biology. ,vol. 268, pp. 570- 584 ,(1997) , 10.1006/JMBI.1997.0987
S.D. Durbin, G. Feher, Studies of crystal growth mechanisms of proteins by electron microscopy Journal of Molecular Biology. ,vol. 212, pp. 763- 774 ,(1990) , 10.1016/0022-2836(90)90235-E
Mitsuo Ataka, Eriko Katoh, Nobuko I. Wakayama, Magnetic orientation as a tool to study the initial stage of crystallization of lysozyme Journal of Crystal Growth. ,vol. 173, pp. 592- 596 ,(1997) , 10.1016/S0022-0248(96)00821-4
F. Rosenberger, S.B. Howard, J.W. Sowers, T.A. Nyce, Temperature dependence of protein solubility — determination and application to crystallization in X-ray capillaries Journal of Crystal Growth. ,vol. 129, pp. 1- 12 ,(1993) , 10.1016/0022-0248(93)90427-X