Expression of pituitary adenylate cyclase-activating polypeptide (PACAP) in the mesencephalic trigeminal nucleus of the rat after transsection of the masseteric nerve

摘要: Pituitary adenylate cyclase-activating polypeptide (PACAP), a member of the VIP (vasoactive intestinal polypeptide) family peptides, has been demonstrated in neurons sensory system. PACAP expression these is sensitive to nerve damages such as crush or axotomy. In present study, mesencephalic trigeminal nucleus rat was examined after transsection main trunk masseteric nerve. The primary are considered have purely proprioceptive functions. By quantitative situ hybridization using [35S]cRNA probe, an increase mRNA observed on side ipsilateral already 3 h and reached peak 24 surgery which levels gradually decreased during next 14 days. A low constant could be seen contralateral transsection. immunoreactivity 18 h, specific monoclonal antibody. Co-existence with NPY galanin 7 days Analysis by radioimmunoassay transsected normal stumps revealed PACAP-38 proximal compared (15.3 vs. 6.1 pmol/g wt). results suggest that role early phase adaptation injury neurons.