Identification of Suitable Reference Genes for Gene Expression Studies of Shoulder Instability

摘要: Shoulder instability is a common shoulder injury, and patients present with plastic deformation of the glenohumeral capsule. Gene expression analysis may be useful tool for increasing general understanding capsule deformation, reverse-transcription quantitative polymerase chain reaction (RT-qPCR) has become an effective method such studies. Although RT-qPCR highly sensitive specific, it requires use suitable reference genes data normalization to guarantee meaningful reproducible results. In study, we evaluated suitability set using samples from capsules individuals without instability. We analyzed six commonly used (ACTB, B2M, GAPDH, HPRT1, TBP TFRC) in antero-inferior, antero-superior posterior portions cases controls. The stability candidate gene was determined four software packages: NormFinder, geNorm, BestKeeper DataAssist. Overall, HPRT1 best single gene, B2M composed pair different groups, including simultaneous all tissue samples. GenEx identify optimal number demonstrated that accumulated standard deviation resulting 2 similar 3 or more genes. To combination genes, COL1A1. combinations yielded variable normalized quantities, relative quantities within sample groups were confirmed no obvious differences observed when 2, 4 Consequently, stable normalization, especially reliable evaluating by RT-qPCR.