Allelic loss on chromosome 3p21.3 and promoter hypermethylation of semaphorin 3B in non-small cell lung cancer.
作者： Larry R. Kaiser , Ayumi Matsuyama , Hansjuerg Alder , Noel N. Williams , Tamotsu Kuroki
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摘要: The aim of this study was to evaluate the promoter methylation status and loss heterozygosity (LOH) SEMA3B in non-small cell lung cancers (NSCLCs). We analyzed semaphorin 3B (SEMA3B) LOH at 3p21.3 eight NSCLC lines 27 primary tumors. Hypermethylation found 50% 41% tumors studied. presence hypermethylation statistically associated with expression both (P = 0.02) < 0.01). There no correlation between tumor stage. On other hand, significant 0.02). Notably, 7 8 showed absence expression. Treatment 5-AZAC restored line. These results indicate that gene alterations may play a important role malignant transformation via two-hit mechanism, including epigenetic changes allelic loss, for suppressor inactivation.
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