Preferential Inactivation of the p53 Tumor Suppressor Pathway and Lack of EGFR Amplification Distinguish de novo High Grade Pediatric Astrocytomas from de novo Adult Astrocytomas

摘要: Classification of high grade astrocytomas children into genetic subtypes similar to the adult remains be defined. Here we report an extensive characterization 29 pediatric astrocytomas, 7 WHO III and 22 IV, for alterations frequently observed in occurring either p53/MDM2/p14ARF or Rb/CDK4/p16INK4a tumor suppressor pathways. In addition, have assessed contribution EGFR overexpression amplification LOH chromosome 10, two commonly associated with development de novo glioblastoma their roles childhood. Our results suggest major differences pathway(s) leading formation compared those adult. findings show preferential inactivation p53 pathway > 95% versus Rb < 25% same tumors. lack gene one-third glioblastomas. Since drug treatments therapy strategies exploit specific cells, our important implications future astrocytomas.