Parathyroid hormone inhibits immunoglobulin production without affecting cell growth in human B cells

摘要: Abstract The effect of parathyroid hormone (PTH) on immunoglobulin (Ig) production and proliferation in the human B-cell lines CBL, SKW, CESS was studied. PTH inhibited Ig from all a dose-dependent manner during 5 days culture. As little as 0.1 ng/ml inhibitory. also cell stimulated by vasoactive intestinal peptide (VIP), interleukin 2 (IL-2), IL-6. This inhibition not due to decreased growth since affected viability always greater than 98%. In contrast PTH, inactivated or triiodothyronine failed affect production. Inhibition blocked anti-PTH serum, but control serum. Of various cytokines tested, IL-4 reduced PTH-induced production, whereas other cytokines, including IL-1β, IL-3, IL-5, interferon α (IFN-α), IFN-γ, granulocyte-macrophage colony-stimulating factor (GM-CSF), do so. reducing anti-IL-4 antibody antibody. Moreover, IFN-α GM-CSF, overcame IL-4. IgG, IgM, IgA tonsillar B cells with Staphylococcus aureus Cowan strain I (SAC) IL-6 without affecting proliferation. These results indicate that, addition its regulatory calcium metabolism, acts an immunoregulatory factor, that it interacts cytokine,