Studies on ether phospholipids

摘要: Abstract A new method for ether phospholipid analysis has been devised, based on the selective destruction of diacyl phospholipids by guinea pig phospholipase A1 and plasmalogens acidolysis. The paper describes optimal conditions allowing a specific degradation enzyme(s). This requires incubation total lipid extract in presence 2.4 mM sodium deoxycholate, at pH 8.0, temperature 42° C. As shown with various radioactive markers, all become degraded, whereas sphingomyelin remain refractory to attack. Phospholipids are then separated bidimensional thin-layer chromatography involving exposure plates HCl fumes between two runs, order hydrolyse plasmalogens. Selectivity both hydrolytic procedures is further demonstrated upon acetyl diacylglycerol derived from phospholipids. Various can thus be determined phosphorus measurement using as an internal standard. By this way, it that Krebs II cells present very high content species (around 25% total). Among these, about 50% alkyl forms ethanolamine phosphoglycerides, value reaches 70% choline phosphoglycerides.