Amino Acid Control of Messenger Ribonucleic Acid Synthesis in Bacillus subtilis

摘要: Abstract We have characterized the response of Bacillus subtilis cells to amino acid starvation, in regard total instantaneous rates RNA synthesis, and levels guanine nucleotides thought be involved mechanisms control a different prokaryote (Escherichia coli). find, agreement with earlier studies, that formation stable species comprises only 16% normal rate synthesis B. subtilis. During arginine is reduced by 65 77%, showing messenger substantially inhibited: smallest reduction compatible our data 58%. A this magnitude consistent results several other briefly reviewed, both E. coli conclude pertains transcription per se, i.e. unstable molecules. Arginine starvation elicits sudden accumulation regulatory MS I (ppGpp) II, an equally contraction GTP pool. Thus, involvement mechanism seems common prokaryotes as divergent general acid-starved may due direct inhibition nucleotides, or diminished supply GTP, both.