Ab initio structure determination of nanocrystals of organic pharmaceutical compounds by electron diffraction at room temperature using a Timepix quantum area direct electron detector

摘要: Until recently, structure determination by transmission electron microscopy of beam-sensitive three-dimensional nanocrystals required diffraction tomography data collection at liquid-nitrogen temperature, in order to reduce radiation damage. Here it is shown that the novel Timepix detector combines a high dynamic range with very signal-to-noise ratio and single-electron sensitivity, enabling ab initio phasing organic compounds. Low-dose (∼0.013 e− A−2 s−1) were collected room temperature rotation method. It was ascertained sufficient quality for solution using direct methods software developed X-ray crystallography (XDS, SHELX) (ADT3D/PETS, SIR2014).