A novel pathway for regulation of glucose-dependent insulinotropic polypeptide (GIP) receptor expression in beta cells.
作者： Francis C. Lynn , Stephen A. Thompson , J. Andrew Pospisilik , Jan A. Ehses , Simon A. Hinke
关键词:
摘要: SPECIFIC AIMGlucose-dependent insulinotropic polypeptide (GIP) is secreted postprandially and acts in concert with glucose to stimulate insulin secretion from the pancreas. In type 2 diabetic patients animal models of disease where there persistent hyperglycemia, ability GIP severely abrogated due a decreased expression receptors on pancreatic β cell. Here we set out define mechanisms by which (GIPR) down-regulation could occur diabetes.PRINCIPAL FINDINGS1. Glucose down-regulates receptor concentration- time-dependent mannerGlucose (25 mM) was able significantly reduce GIPR mRNA 64 ± 7% 28 6% basal levels INS(832/13) cells after 6 24 h, respectively. Furthermore, h incubation 11 mM caused significant, 36 7%, reduction these cells. Saturation binding analyses showed marked, statistically significant decrease amount ...
elsevier.com
fasebj.org参考文章(41)
James P. KEHRER, Shyam S. BISWAL, Eunhye LA, Philippe THUILLIER, Kaushik DATTA, Susan M. FISCHER, John P. VANDEN HEUVEL, Inhibition of peroxisome-proliferator-activated receptor (PPAR)α by MK886 Biochemical Journal. ,vol. 356, pp. 899- 906 ,(2001) , 10.1042/0264-6021:3560899
A D Blake, N S Hayes, E E Slater, C D Strader, Insulin receptor desensitization correlates with attenuation of tyrosine kinase activity, but not of receptor endocytosis Biochemical Journal. ,vol. 245, pp. 357- 364 ,(1987) , 10.1042/BJ2450357
Willard M. Freeman, Stephen J. Walker, Kent E. Vrana, Quantitative RT-PCR: pitfalls and potential. BioTechniques. ,vol. 26, pp. 112- 125 ,(1999) , 10.2144/99261RV01
Y.-T. Zhou, M. Shimabukuro, M.-Y. Wang, Y. Lee, M. Higa, J. L. Milburn, C. B. Newgard, R. H. Unger, Role of peroxisome proliferator-activated receptor α in disease of pancreatic β cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 8898- 8903 ,(1998) , 10.1073/PNAS.95.15.8898
M A Nauck, M M Heimesaat, C Orskov, J J Holst, R Ebert, W Creutzfeldt, Preserved incretin activity of glucagon-like peptide 1 [7-36 amide] but not of synthetic human gastric inhibitory polypeptide in patients with type-2 diabetes mellitus. Journal of Clinical Investigation. ,vol. 91, pp. 301- 307 ,(1993) , 10.1172/JCI116186
T J Kieffer, C H McIntosh, R A Pederson, Degradation of glucose-dependent insulinotropic polypeptide and truncated glucagon-like peptide 1 in vitro and in vivo by dipeptidyl peptidase IV Endocrinology. ,vol. 136, pp. 3585- 3596 ,(1995) , 10.1210/ENDO.136.8.7628397
Andrea Trümper, Katja Trümper, Heidi Trusheim, Rudolf Arnold, Burkhard Göke, Dieter Hörsch, Glucose-Dependent Insulinotropic Polypeptide Is a Growth Factor for β (INS-1) Cells by Pleiotropic Signaling Molecular Endocrinology. ,vol. 15, pp. 1559- 1570 ,(2001) , 10.1210/MEND.15.9.0688
Philippe Gervois, Inés Pineda Torra, Giulia Chinetti, Thilo Grötzinger, Guillaume Dubois, Jean-Charles Fruchart, Jamila Fruchart-Najib, Eran Leitersdorf, Bart Staels, A Truncated Human Peroxisome Proliferator-Activated Receptor α Splice Variant with Dominant Negative Activity Molecular Endocrinology. ,vol. 13, pp. 1535- 1549 ,(1999) , 10.1210/MEND.13.9.0341
W.-G. Ding, J. Gromada, Protein kinase A-dependent stimulation of exocytosis in mouse pancreatic beta-cells by glucose-dependent insulinotropic polypeptide. Diabetes. ,vol. 46, pp. 615- 621 ,(1997) , 10.2337/DIAB.46.4.615
Christopher H. S. McIntosh, Irene Bremsak, Francis C. Lynn, Ruth Gill, Simon A. Hinke, Richard Gelling, Cuilan Nian, Gary McKnight, Stephen Jaspers, Raymond A. Pederson, GLUCOSE-DEPENDENT INSULINOTROPIC POLYPEPTIDE STIMULATION OF LIPOLYSIS IN DIFFERENTIATED 3T3-L1 CELLS : WORTMANNIN-SENSITIVE INHIBITION BY INSULIN Endocrinology. ,vol. 140, pp. 398- 404 ,(1999) , 10.1210/ENDO.140.1.6464