Isolation of Ethyl Methanesulfonate-Induced Gametophytic Mutants in Arabidopsis thaliana by a Segregation Distortion Assay Using the Multimarker Chromosome 1

摘要: The life cycle of plants comprises two alternating generations, the diploid sporophyte (spore-bearing plant) and haploid gametophyte (gamete-bearing plant). In contrast to animals, postmeiotic cells give rise organisms whose function is produce gametes mediate fertilization. Analysis development has been hampered by difficulty identifying haplo-phase-specific mutants in conventional mutagenesis screens. Here we use a genetic strategy that based on segregation distortion nearby visible markers screen for EMS-induced gametophytic Arabidopsis thaliana. Using multiple marker chromosome mm1 have isolated seven lines displayed an altered markers. Reciprocal backcrosses these showed marked reduction transmission male and/or female gametes. Phenotypic analysis revealed different aspects either or were affected. Three specific arrests during pollen development. One line was specifically defective tube elongation. variable defects both