Interferon‐γ differentially modulates the release of cytokines and chemokines in lipopolysaccharide‐ and pneumococcal cell wall‐stimulated mouse microglia and macrophages

摘要: During bacterial infections of the CNS, activated microglia could support leucocyte recruitment to brain through synthesis cyto- and chemokines. In turn, invading leucocytes may feedback on microglial cells influence their chemokine release pattern. Here, we analyzed capacity interferon-gamma (IFNgamma) serve as such a leucocyte-to-microglia signal. Production chemokines was stimulated in mouse cultures by treatments with lipopolysaccharide (LPS) from Gram-negative Escherichia coli or cell walls Gram-positive Streptococcus pneumoniae (PCW). IFNgamma presence during stimulation (0.1-100 ng/mL) modulated patterns LPS- PCW-induced dose-dependent, potent complex manner. While amounts TNFalpha IL-6 remained nearly unchanged, enhanced production IL-12, MCP-1 RANTES, but attenuated that KC, MIP-1alpha MIP-2. Release modulation obtained preincubation (treatment before LPS PCW administration), coincubation even delayed addition an ongoing stimulation. Together changes observed for under would shift chemoattractive profile favouring neutrophils preferential attraction monocytes T lymphocyte populations--as actually seen course meningitis. The findings view major intrinsic source instant variety suggest leucocyte-derived potentially regulate