Bilaterian phylogeny based on analyses of a region of the sodium-potassium ATPase beta-subunit gene.

摘要: Molecular investigations of deep-level relationships within and among the animal phyla have been hampered by a lack slowly evolving genes that are amenable to study molecular systematists. To provide new data for use in metazoan phylogenetic studies, primers were developed amplify 1.3-kb region α subunit nuclear-encoded sodium–potassium ATPase gene from 31 bilaterians representing several phyla. Maximum parsimony, maximum likelihood, Bayesian analyses these sequences (combined with 23 taxa downloaded GenBank) yield congruent trees corroborate recent findings based on other sets (e.g., 18S ribosomal RNA gene). The ATPase-based support monophyly clades (including Lophotrochozoa, form Ecdysozoa, Vertebrata, Mollusca, Bivalvia, Gastropoda, Arachnida, Hexapoda, Coleoptera, Diptera) but do not Deuterostomia, Arthropoda, or Nemertea. Parametric bootstrapping tests reject Arthropoda Nemertea unable deuterostome monophyly. Overall, α-subunit appears be useful studies phylogeny.